pspax2 plasmid constructs Search Results


pspax2 plasmid constructs  (Addgene inc)
93
Addgene inc pspax2 plasmid constructs
Pspax2 Plasmid Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspax2+plasmid+constructs/PUMA+Frag1-Luc+(Plasmid+%2316591)/pmc09923402__DataSheet_1-0-11-36
Average 93 stars, based on 1 article reviews
pspax2 plasmid constructs - by Bioz Stars, 2026-09
93/100 stars
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envelope plasmid pvsvg  (Addgene inc)
98
Addgene inc envelope plasmid pvsvg
Envelope Plasmid Pvsvg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspax2+plasmid+constructs/psPAX2+(Plasmid+%2312260)/pm40102981-65-16-19
Average 98 stars, based on 1 article reviews
envelope plasmid pvsvg - by Bioz Stars, 2026-09
98/100 stars
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lentiviral packaging vector pspax2  (Addgene inc)
98
Addgene inc lentiviral packaging vector pspax2
Lentiviral Packaging Vector Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspax2+plasmid+constructs/pMD2%2EG+(Plasmid+%2312259)/bio_rxiv__2024__10__15__618475-99-5-10
Average 98 stars, based on 1 article reviews
lentiviral packaging vector pspax2 - by Bioz Stars, 2026-09
98/100 stars
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pspax2  (Addgene inc)
92
Addgene inc pspax2
Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspax2+plasmid+constructs/pSB819-PKR-gib+(Plasmid+%2320031)/pm25037569-199-31-40
Average 92 stars, based on 1 article reviews
pspax2 - by Bioz Stars, 2026-09
92/100 stars
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pspax2  (Broad Institute Inc)
90
Broad Institute Inc pspax2
Pspax2, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspax2+plasmid+constructs/pspax2/pm25666808-273-50-74
Average 90 stars, based on 1 article reviews
pspax2 - by Bioz Stars, 2026-09
90/100 stars
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recombinant dna reagent atf4  (Addgene inc)
93
Addgene inc recombinant dna reagent atf4
Figure 4. Use of activating transcription factor 4 <t>(ATF4)</t> knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and <t>DNA-binding</t> domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-
Recombinant Dna Reagent Atf4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspax2+plasmid+constructs/ATF4+1%3A+Mouse+ATF4+(CHOP11%2FcATF)-WT+(Plasmid+%2321845)/10__7554_slash_elife__63326-275-110-118
Average 93 stars, based on 1 article reviews
recombinant dna reagent atf4 - by Bioz Stars, 2026-09
93/100 stars
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packaging construct pspax2  (Addgene inc)
93
Addgene inc packaging construct pspax2
Figure 4. Use of activating transcription factor 4 <t>(ATF4)</t> knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and <t>DNA-binding</t> domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-
Packaging Construct Pspax2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspax2+plasmid+constructs/CMV-GFP-NMHC+II-B+(Plasmid+%2311348)/pm37029141-248-3-19
Average 93 stars, based on 1 article reviews
packaging construct pspax2 - by Bioz Stars, 2026-09
93/100 stars
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shrna constructs  (Addgene inc)
96
Addgene inc shrna constructs
Figure 4. Use of activating transcription factor 4 <t>(ATF4)</t> knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and <t>DNA-binding</t> domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-
Shrna Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspax2+plasmid+constructs/shRNA+(Plasmid+%2355783)/pm32272059-307-15-25
Average 96 stars, based on 1 article reviews
shrna constructs - by Bioz Stars, 2026-09
96/100 stars
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pspax2 packaging construct  (PlasmidFactory gmbh)
90
PlasmidFactory gmbh pspax2 packaging construct
Figure 4. Use of activating transcription factor 4 <t>(ATF4)</t> knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and <t>DNA-binding</t> domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-
Pspax2 Packaging Construct, supplied by PlasmidFactory gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspax2+plasmid+constructs/pspax2+packaging+construct/pmc02642742-144-40-43
Average 90 stars, based on 1 article reviews
pspax2 packaging construct - by Bioz Stars, 2026-09
90/100 stars
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gene exp btn3a1 hs01063368 m1  (Thermo Fisher)
86
Thermo Fisher gene exp btn3a1 hs01063368 m1
Figure 4. Use of activating transcription factor 4 <t>(ATF4)</t> knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and <t>DNA-binding</t> domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-
Gene Exp Btn3a1 Hs01063368 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pspax2+plasmid+constructs/Gene+Exp%2E+BTN3A1%2C+Hs01063368_m1/pm34260935-257-10-13
Average 86 stars, based on 1 article reviews
gene exp btn3a1 hs01063368 m1 - by Bioz Stars, 2026-09
86/100 stars
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Image Search Results


Figure 4. Use of activating transcription factor 4 (ATF4) knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and DNA-binding domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-

Journal: eLife

Article Title: The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals

doi: 10.7554/elife.63326

Figure Lengend Snippet: Figure 4. Use of activating transcription factor 4 (ATF4) knockout cells and a rapamycin-resistant ATF4 to validate ATF4 targets regulated by mechanistic target of rapamycin complex 1 signaling. (A) Schematic of ATF4 transcript, including upstream open reading frames (uORFs), coding sequence (CDS), and DNA-binding domain (DNABD), highlighting location of CRISPRn guides biallelic location of ATF4 mutations generated in Tsc2-/-

Article Snippet: DOI: https://doi.org/10.7554/eLife.63326 19 of 33 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Transfected construct (mouse) siAtf4 GE Life Sciences/ Dharmacon L-042737-01-0020 Transfected construct (mouse) siRheb GE Life Sciences/ Dharmacon L-057044-00-0020 Transfected construct (mouse) siRhebL1 GE Life Sciences/ Dharmacon L-056074-01-0020 Transfected construct (mouse) siTsc2 GE Life Sciences/ Dharmacon L-047050-00-0020 Transfected construct (mouse) siC/ebpa GE Life Sciences/ Dharmacon L-040561-00-0005 Transfected construct (mouse) siC/ebpb GE Life Sciences/ Dharmacon L-043110-00-0005 Transfected construct (mouse) siC/ebpd GE Life Sciences/ Dharmacon L-060294-01-0005 Transfected construct (mouse) siC/ebpg GE Life Sciences/ Dharmacon L-065627-00-0005 Transfected construct (human) siATF4 GE Life Sciences/ Dharmacon L-005125-00-0020 Sequenced-based reagent qPCR primers IDT See table in Materials and methods Recombinant DNA reagent ATF4 (cDNA amplified from plasmid) Addgene RRID:Addgene_21845 Recombinant DNA reagent Pspax2 (plasmid) Addgene RRID:Addgene_12260 Recombinant DNA reagent Pmd2.G (plasmid) Addgene RRID:Addgene_12259 Recombinant DNA reagent pSpCas9n(BB) 2A-GFP (PX461) (plasmid) Addgene RRID:Addgene_48140 Recombinant DNA reagent pTRIPZ (plasmid) PMID:27088857 Alex Toker (Beth Israel Deaconess Medical Center) Recombinant DNA reagent GFP (cDNA amplified from plasmid) Addgene RRID:Addgene_19319 Recombinant DNA reagent SLC7A11 (cDNA amplified from plasmid) PMID:29259101 Alex Toker (Beth Israel Deaconess Medical Center) Recombinant DNA reagent pBabe hygro IRES-TSC2 PMID:15150095 David Kwiatkowski (Brigham and Women’s Hospital) Sequenced-based reagent CRISPR-Cas9n guides for KO of ATF4 IDT CACCGGAGG TGGAGGGGCTATGCT; AAACAGCATAGCCCC TCCACCTCC; CACCGACAATCTGCCTTC TCCAGG; AAACCC TGGAGAAGGCAGATTG TC Sequenced-based reagent Sequencing primers for Atf4-/- cell lines IDT TCGATGCTCTGTTTCGAA TG; CTTCTTCCCCC TTGCCTTAC Continued on next page Torrence et al. eLife 2021;10:e63326.

Techniques: Knock-Out, Sequencing, Binding Assay, Generated

Figure 5. Activation of activating transcription factor 4 (ATF4) contributes to the induction of protein synthesis downstream of mechanistic target of rapamycin complex 1. (A, B) Representative autoradiogram and immunoblot of wild-type (WT) and Tsc2-/- mouse embryo fibroblasts (MEFs) transfected with siRNAs targeting Atf4 or Rheb1 and Rhebl1 or non-targeting controls (siCT) and serum-deprived for 16 hr with a pulse label of [35S]-methionine for the final 20 min (A) and quantified in (B). Biological triplicates from a representative experiment are shown in (A). (B) is graphed as mean ± SEM from Figure 5 continued on next page

Journal: eLife

Article Title: The mTORC1-mediated activation of ATF4 promotes protein and glutathione synthesis downstream of growth signals

doi: 10.7554/elife.63326

Figure Lengend Snippet: Figure 5. Activation of activating transcription factor 4 (ATF4) contributes to the induction of protein synthesis downstream of mechanistic target of rapamycin complex 1. (A, B) Representative autoradiogram and immunoblot of wild-type (WT) and Tsc2-/- mouse embryo fibroblasts (MEFs) transfected with siRNAs targeting Atf4 or Rheb1 and Rhebl1 or non-targeting controls (siCT) and serum-deprived for 16 hr with a pulse label of [35S]-methionine for the final 20 min (A) and quantified in (B). Biological triplicates from a representative experiment are shown in (A). (B) is graphed as mean ± SEM from Figure 5 continued on next page

Article Snippet: DOI: https://doi.org/10.7554/eLife.63326 19 of 33 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Transfected construct (mouse) siAtf4 GE Life Sciences/ Dharmacon L-042737-01-0020 Transfected construct (mouse) siRheb GE Life Sciences/ Dharmacon L-057044-00-0020 Transfected construct (mouse) siRhebL1 GE Life Sciences/ Dharmacon L-056074-01-0020 Transfected construct (mouse) siTsc2 GE Life Sciences/ Dharmacon L-047050-00-0020 Transfected construct (mouse) siC/ebpa GE Life Sciences/ Dharmacon L-040561-00-0005 Transfected construct (mouse) siC/ebpb GE Life Sciences/ Dharmacon L-043110-00-0005 Transfected construct (mouse) siC/ebpd GE Life Sciences/ Dharmacon L-060294-01-0005 Transfected construct (mouse) siC/ebpg GE Life Sciences/ Dharmacon L-065627-00-0005 Transfected construct (human) siATF4 GE Life Sciences/ Dharmacon L-005125-00-0020 Sequenced-based reagent qPCR primers IDT See table in Materials and methods Recombinant DNA reagent ATF4 (cDNA amplified from plasmid) Addgene RRID:Addgene_21845 Recombinant DNA reagent Pspax2 (plasmid) Addgene RRID:Addgene_12260 Recombinant DNA reagent Pmd2.G (plasmid) Addgene RRID:Addgene_12259 Recombinant DNA reagent pSpCas9n(BB) 2A-GFP (PX461) (plasmid) Addgene RRID:Addgene_48140 Recombinant DNA reagent pTRIPZ (plasmid) PMID:27088857 Alex Toker (Beth Israel Deaconess Medical Center) Recombinant DNA reagent GFP (cDNA amplified from plasmid) Addgene RRID:Addgene_19319 Recombinant DNA reagent SLC7A11 (cDNA amplified from plasmid) PMID:29259101 Alex Toker (Beth Israel Deaconess Medical Center) Recombinant DNA reagent pBabe hygro IRES-TSC2 PMID:15150095 David Kwiatkowski (Brigham and Women’s Hospital) Sequenced-based reagent CRISPR-Cas9n guides for KO of ATF4 IDT CACCGGAGG TGGAGGGGCTATGCT; AAACAGCATAGCCCC TCCACCTCC; CACCGACAATCTGCCTTC TCCAGG; AAACCC TGGAGAAGGCAGATTG TC Sequenced-based reagent Sequencing primers for Atf4-/- cell lines IDT TCGATGCTCTGTTTCGAA TG; CTTCTTCCCCC TTGCCTTAC Continued on next page Torrence et al. eLife 2021;10:e63326.

Techniques: Activation Assay, Western Blot, Transfection